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2011 bacteria citrobacter freundii atcc 33128 american type culture collection  (ATCC)
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ATCC 2011 bacteria citrobacter freundii atcc 33128 american type culture collection
2011 Bacteria Citrobacter Freundii Atcc 33128 American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tag72  (Novus Biologicals)
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Novus Biologicals tag72
Fig. 2 | Membrane-bound IL-12 engineered <t>TAG72-CAR</t> T cells induce higher IFNγ, T-cell expansion, and anti-tumor activity in vitro. a–c Tumor cell killing of OV90 cells by TAG72-CAR T cells (E:T = 1:20) with the addition of varying con- centrations of anti-IFNγR1 blocking antibody, isotype control, and recombinant human IL-12 cytokine measured by xCELLigence over 10 days (a, b). n = 2/group at each timepoint. Data are presented as mean values ±SD. At day 10, IFNγ levels in supernatants were quantified by ELISA (c). n = 2/group, representative of two independent experiments. Data are presented as mean values ±SD. d Illustration of TAG72-CAR/mbIL12 T cell. e Flow cytometric analysis of surface or intracellular expression of mbIL12 in TAG72-CAR T cells stimulated with varying concentrations of plate-bound TAG72. n = 2/group, representative of two independent experi- ments. Data are presented as mean values ±SD. f Intracellular flow cytometric analysis of phosphorylated STAT3 (pSTAT3, pY705) (left) and pSTAT4 (right) in
Tag72, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c freundii 33128 1e5  (ATCC)
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ATCC c freundii 33128 1e5
Fig. 2 | Membrane-bound IL-12 engineered <t>TAG72-CAR</t> T cells induce higher IFNγ, T-cell expansion, and anti-tumor activity in vitro. a–c Tumor cell killing of OV90 cells by TAG72-CAR T cells (E:T = 1:20) with the addition of varying con- centrations of anti-IFNγR1 blocking antibody, isotype control, and recombinant human IL-12 cytokine measured by xCELLigence over 10 days (a, b). n = 2/group at each timepoint. Data are presented as mean values ±SD. At day 10, IFNγ levels in supernatants were quantified by ELISA (c). n = 2/group, representative of two independent experiments. Data are presented as mean values ±SD. d Illustration of TAG72-CAR/mbIL12 T cell. e Flow cytometric analysis of surface or intracellular expression of mbIL12 in TAG72-CAR T cells stimulated with varying concentrations of plate-bound TAG72. n = 2/group, representative of two independent experi- ments. Data are presented as mean values ±SD. f Intracellular flow cytometric analysis of phosphorylated STAT3 (pSTAT3, pY705) (left) and pSTAT4 (right) in
C Freundii 33128 1e5, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c freundii 33128 1e7  (ATCC)
91
ATCC c freundii 33128 1e7
Fig. 2 | Membrane-bound IL-12 engineered <t>TAG72-CAR</t> T cells induce higher IFNγ, T-cell expansion, and anti-tumor activity in vitro. a–c Tumor cell killing of OV90 cells by TAG72-CAR T cells (E:T = 1:20) with the addition of varying con- centrations of anti-IFNγR1 blocking antibody, isotype control, and recombinant human IL-12 cytokine measured by xCELLigence over 10 days (a, b). n = 2/group at each timepoint. Data are presented as mean values ±SD. At day 10, IFNγ levels in supernatants were quantified by ELISA (c). n = 2/group, representative of two independent experiments. Data are presented as mean values ±SD. d Illustration of TAG72-CAR/mbIL12 T cell. e Flow cytometric analysis of surface or intracellular expression of mbIL12 in TAG72-CAR T cells stimulated with varying concentrations of plate-bound TAG72. n = 2/group, representative of two independent experi- ments. Data are presented as mean values ±SD. f Intracellular flow cytometric analysis of phosphorylated STAT3 (pSTAT3, pY705) (left) and pSTAT4 (right) in
C Freundii 33128 1e7, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c freundii 33128 1e7/product/ATCC
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citrobacter freundii  (ATCC)
91
ATCC citrobacter freundii
Fig. 2 | Membrane-bound IL-12 engineered <t>TAG72-CAR</t> T cells induce higher IFNγ, T-cell expansion, and anti-tumor activity in vitro. a–c Tumor cell killing of OV90 cells by TAG72-CAR T cells (E:T = 1:20) with the addition of varying con- centrations of anti-IFNγR1 blocking antibody, isotype control, and recombinant human IL-12 cytokine measured by xCELLigence over 10 days (a, b). n = 2/group at each timepoint. Data are presented as mean values ±SD. At day 10, IFNγ levels in supernatants were quantified by ELISA (c). n = 2/group, representative of two independent experiments. Data are presented as mean values ±SD. d Illustration of TAG72-CAR/mbIL12 T cell. e Flow cytometric analysis of surface or intracellular expression of mbIL12 in TAG72-CAR T cells stimulated with varying concentrations of plate-bound TAG72. n = 2/group, representative of two independent experi- ments. Data are presented as mean values ±SD. f Intracellular flow cytometric analysis of phosphorylated STAT3 (pSTAT3, pY705) (left) and pSTAT4 (right) in
Citrobacter Freundii, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2 | Membrane-bound IL-12 engineered TAG72-CAR T cells induce higher IFNγ, T-cell expansion, and anti-tumor activity in vitro. a–c Tumor cell killing of OV90 cells by TAG72-CAR T cells (E:T = 1:20) with the addition of varying con- centrations of anti-IFNγR1 blocking antibody, isotype control, and recombinant human IL-12 cytokine measured by xCELLigence over 10 days (a, b). n = 2/group at each timepoint. Data are presented as mean values ±SD. At day 10, IFNγ levels in supernatants were quantified by ELISA (c). n = 2/group, representative of two independent experiments. Data are presented as mean values ±SD. d Illustration of TAG72-CAR/mbIL12 T cell. e Flow cytometric analysis of surface or intracellular expression of mbIL12 in TAG72-CAR T cells stimulated with varying concentrations of plate-bound TAG72. n = 2/group, representative of two independent experi- ments. Data are presented as mean values ±SD. f Intracellular flow cytometric analysis of phosphorylated STAT3 (pSTAT3, pY705) (left) and pSTAT4 (right) in

Journal: Nature communications

Article Title: Antigen-dependent IL-12 signaling in CAR T cells promotes regional to systemic disease targeting.

doi: 10.1038/s41467-023-40115-1

Figure Lengend Snippet: Fig. 2 | Membrane-bound IL-12 engineered TAG72-CAR T cells induce higher IFNγ, T-cell expansion, and anti-tumor activity in vitro. a–c Tumor cell killing of OV90 cells by TAG72-CAR T cells (E:T = 1:20) with the addition of varying con- centrations of anti-IFNγR1 blocking antibody, isotype control, and recombinant human IL-12 cytokine measured by xCELLigence over 10 days (a, b). n = 2/group at each timepoint. Data are presented as mean values ±SD. At day 10, IFNγ levels in supernatants were quantified by ELISA (c). n = 2/group, representative of two independent experiments. Data are presented as mean values ±SD. d Illustration of TAG72-CAR/mbIL12 T cell. e Flow cytometric analysis of surface or intracellular expression of mbIL12 in TAG72-CAR T cells stimulated with varying concentrations of plate-bound TAG72. n = 2/group, representative of two independent experi- ments. Data are presented as mean values ±SD. f Intracellular flow cytometric analysis of phosphorylated STAT3 (pSTAT3, pY705) (left) and pSTAT4 (right) in

Article Snippet: Antibodies against CD3 (BD Biosciences, Cat: 563109, Clone: SK7), CD4 (BD Biosciences, Cat: 340443, Clone: SK3), CD8 (BD Biosciences, Cat: 347313, Clone: SK1), CD19 (BDBiosciences, Cat: 557835, Clone: SJ25C1), mouse CD45 (BioLegend, Cat: 103145, Clone: 30-F11), CD45 (BD Biosciences, Cat: 555484, Clone: 2D1), CD69 (BD Biosciences, Cat: 341652, Clone: L78), CD137 (BD Biosciences, Cat: 555956, Clone: 4B4-1), mouse CD137 (Thermofisher, Cat: 25-1371-82, Clone: 17B5), mouse NK1.1 (BioLegend, Cat: 108733, Clone: PK163), mouse PD-1 (Thermofisher, Cat: 69-9985-80, Clone: J43), mouse LAG3 (BioLegend, Cat: 125227, Clone: C9B7W), mouse TIM-3 (BioLegend, Cat: 119704, Clone: RMT323), mouse CD11b (BioLegend, Cat: 101237, Clone: M1/70), CD44 (BioLegend, Cat: 103010, Clone: IM7), CD62L (BioLegend, Cat: 104412, Clone: MEL-14), CD80 (BD Biosciences, Cat: 740130, Clone: 16-10A1), mouse I-A/I-E (MHC Class II) (Thermofisher, Cat: 64-5321-80, Clone: M5/114.15.2), mouse CD274 (PD-L1) (BioLegend, Cat: 124312, Clone: 10 F.962), Ly6C (BioLegend, Cat: 128029, Clone: HK1.4), mouse CD11c (BioLegend, Cat: 117316, Clone: N418), mouse Ly6G (Biolegend, Cat: 127623, Clone: 1A8), mouse CD103 (BioLegend, Cat: 121426, Clone: 2E7), mouse F4/80 (BioLegend, Cat: 123127, Clone: BM8), mouse IL-12/ IL-23 p40 (Thermofisher, Cat: 12-7123-41, Clone:17.8), Ep-CAM/CD326 (BioLegend, Clone: 9C4) (Cat: 324208), human IL-12 p40/p70 (BD Biosciences, Cat: 554575, Clone: C11:5), biotinylated Protein L (GenScript USA, Cat: M00097) (25), TAG72 (Novus Biologicals), Clone, Cat: NBP2-33128, muCC49), Donkey Anti-Rabbit Ig (Invitrogen, Cat: A31573), Goat Anti-Mouse Ig (BD Biosciences, Cat: 550589), and streptavidin (BD Biosciences, Cat: 349023, 1:20 dilution) were used.

Techniques: Membrane, Activity Assay, In Vitro, Blocking Assay, Control, Recombinant, Enzyme-linked Immunosorbent Assay, Expressing

Fig. 3 | Locoregional intraperitoneal delivery of TAG72-CAR/mbIL12 T cells reduces tumor burden and increases regional and systemic CAR T-cell persis- tence in vivo. a Representative bioluminescent flux imaging of i.p. OV90(eGFP/ ffluc) tumor-bearing mice treated i.p. with CD19-CAR, CD19-CAR/mbIL12, TAG72- CAR or TAG72-CAR/mbIL12 T cells. b Quantification of flux (individual mice per group) from OV90(eGFP/ffluc) tumor-bearing mice treated i.p. with CD19-CAR T cells (n = 12/group), CD19-CAR/mbIL12 T cells (n = 12/group), TAG72-CAR T cells (n = 20/group) and TAG72-CAR/mbIL12 T cells (n = 17/group). Combined data are from two independent studies. P value indicates differences between TAG72-CAR and TAG72-CAR/mbIL12 using a two-tailed Student’s t test. c Representative flow cytometric analysis of TAG72-CAR T cells per µL of peritoneal ascites. d Quantification of TAG72-CAR T cells per µL of peritoneal ascites (left) at weeks 2 and 4 post-treatment. n = 2/group per timepoint. Quantification of TAG72-CAR T cells per µL of peripheral blood (right) at weeks 1, 2, and 4 post-treatment. n = 5/ group per timepoint. Data are presented as mean values ±SEM. P values indicate

Journal: Nature communications

Article Title: Antigen-dependent IL-12 signaling in CAR T cells promotes regional to systemic disease targeting.

doi: 10.1038/s41467-023-40115-1

Figure Lengend Snippet: Fig. 3 | Locoregional intraperitoneal delivery of TAG72-CAR/mbIL12 T cells reduces tumor burden and increases regional and systemic CAR T-cell persis- tence in vivo. a Representative bioluminescent flux imaging of i.p. OV90(eGFP/ ffluc) tumor-bearing mice treated i.p. with CD19-CAR, CD19-CAR/mbIL12, TAG72- CAR or TAG72-CAR/mbIL12 T cells. b Quantification of flux (individual mice per group) from OV90(eGFP/ffluc) tumor-bearing mice treated i.p. with CD19-CAR T cells (n = 12/group), CD19-CAR/mbIL12 T cells (n = 12/group), TAG72-CAR T cells (n = 20/group) and TAG72-CAR/mbIL12 T cells (n = 17/group). Combined data are from two independent studies. P value indicates differences between TAG72-CAR and TAG72-CAR/mbIL12 using a two-tailed Student’s t test. c Representative flow cytometric analysis of TAG72-CAR T cells per µL of peritoneal ascites. d Quantification of TAG72-CAR T cells per µL of peritoneal ascites (left) at weeks 2 and 4 post-treatment. n = 2/group per timepoint. Quantification of TAG72-CAR T cells per µL of peripheral blood (right) at weeks 1, 2, and 4 post-treatment. n = 5/ group per timepoint. Data are presented as mean values ±SEM. P values indicate

Article Snippet: Antibodies against CD3 (BD Biosciences, Cat: 563109, Clone: SK7), CD4 (BD Biosciences, Cat: 340443, Clone: SK3), CD8 (BD Biosciences, Cat: 347313, Clone: SK1), CD19 (BDBiosciences, Cat: 557835, Clone: SJ25C1), mouse CD45 (BioLegend, Cat: 103145, Clone: 30-F11), CD45 (BD Biosciences, Cat: 555484, Clone: 2D1), CD69 (BD Biosciences, Cat: 341652, Clone: L78), CD137 (BD Biosciences, Cat: 555956, Clone: 4B4-1), mouse CD137 (Thermofisher, Cat: 25-1371-82, Clone: 17B5), mouse NK1.1 (BioLegend, Cat: 108733, Clone: PK163), mouse PD-1 (Thermofisher, Cat: 69-9985-80, Clone: J43), mouse LAG3 (BioLegend, Cat: 125227, Clone: C9B7W), mouse TIM-3 (BioLegend, Cat: 119704, Clone: RMT323), mouse CD11b (BioLegend, Cat: 101237, Clone: M1/70), CD44 (BioLegend, Cat: 103010, Clone: IM7), CD62L (BioLegend, Cat: 104412, Clone: MEL-14), CD80 (BD Biosciences, Cat: 740130, Clone: 16-10A1), mouse I-A/I-E (MHC Class II) (Thermofisher, Cat: 64-5321-80, Clone: M5/114.15.2), mouse CD274 (PD-L1) (BioLegend, Cat: 124312, Clone: 10 F.962), Ly6C (BioLegend, Cat: 128029, Clone: HK1.4), mouse CD11c (BioLegend, Cat: 117316, Clone: N418), mouse Ly6G (Biolegend, Cat: 127623, Clone: 1A8), mouse CD103 (BioLegend, Cat: 121426, Clone: 2E7), mouse F4/80 (BioLegend, Cat: 123127, Clone: BM8), mouse IL-12/ IL-23 p40 (Thermofisher, Cat: 12-7123-41, Clone:17.8), Ep-CAM/CD326 (BioLegend, Clone: 9C4) (Cat: 324208), human IL-12 p40/p70 (BD Biosciences, Cat: 554575, Clone: C11:5), biotinylated Protein L (GenScript USA, Cat: M00097) (25), TAG72 (Novus Biologicals), Clone, Cat: NBP2-33128, muCC49), Donkey Anti-Rabbit Ig (Invitrogen, Cat: A31573), Goat Anti-Mouse Ig (BD Biosciences, Cat: 550589), and streptavidin (BD Biosciences, Cat: 349023, 1:20 dilution) were used.

Techniques: In Vivo, Imaging, Two Tailed Test

Fig. 6 | TAG72-CAR/mbIL12 T-cell therapy induces TME modifications in ovarian cancer peritoneal metastases in an immune-competent syngeneic mouse model. a Representative H&E and CD3 IHC in solid tumor masses collected from the upper omental region of i.p. ID8-mSTn tumor-bearing mice treated with indicated T cells. Scale bar = 100 µm. b Representative flow cytometric analysis of tumor cells (CD3-CD45- double negative) in peritoneal ascites. c Quantification of tumor cells (CD3−CD45−double negative) and d immune subsets (CD45+, CD3+, CD11b+, and NK+) as cells/mL in peritoneal ascites. e Representative flow cyto- metric analysis of percent CAR T cells (CD3+ CD19t+) and f quantification counts of CD4+ and CD8+ CAR T cells/mL in peritoneal ascites. g Quantification of mean fluorescent intensity (MFI) of CD137+ in CAR T cells in peritoneal ascites.

Journal: Nature communications

Article Title: Antigen-dependent IL-12 signaling in CAR T cells promotes regional to systemic disease targeting.

doi: 10.1038/s41467-023-40115-1

Figure Lengend Snippet: Fig. 6 | TAG72-CAR/mbIL12 T-cell therapy induces TME modifications in ovarian cancer peritoneal metastases in an immune-competent syngeneic mouse model. a Representative H&E and CD3 IHC in solid tumor masses collected from the upper omental region of i.p. ID8-mSTn tumor-bearing mice treated with indicated T cells. Scale bar = 100 µm. b Representative flow cytometric analysis of tumor cells (CD3-CD45- double negative) in peritoneal ascites. c Quantification of tumor cells (CD3−CD45−double negative) and d immune subsets (CD45+, CD3+, CD11b+, and NK+) as cells/mL in peritoneal ascites. e Representative flow cyto- metric analysis of percent CAR T cells (CD3+ CD19t+) and f quantification counts of CD4+ and CD8+ CAR T cells/mL in peritoneal ascites. g Quantification of mean fluorescent intensity (MFI) of CD137+ in CAR T cells in peritoneal ascites.

Article Snippet: Antibodies against CD3 (BD Biosciences, Cat: 563109, Clone: SK7), CD4 (BD Biosciences, Cat: 340443, Clone: SK3), CD8 (BD Biosciences, Cat: 347313, Clone: SK1), CD19 (BDBiosciences, Cat: 557835, Clone: SJ25C1), mouse CD45 (BioLegend, Cat: 103145, Clone: 30-F11), CD45 (BD Biosciences, Cat: 555484, Clone: 2D1), CD69 (BD Biosciences, Cat: 341652, Clone: L78), CD137 (BD Biosciences, Cat: 555956, Clone: 4B4-1), mouse CD137 (Thermofisher, Cat: 25-1371-82, Clone: 17B5), mouse NK1.1 (BioLegend, Cat: 108733, Clone: PK163), mouse PD-1 (Thermofisher, Cat: 69-9985-80, Clone: J43), mouse LAG3 (BioLegend, Cat: 125227, Clone: C9B7W), mouse TIM-3 (BioLegend, Cat: 119704, Clone: RMT323), mouse CD11b (BioLegend, Cat: 101237, Clone: M1/70), CD44 (BioLegend, Cat: 103010, Clone: IM7), CD62L (BioLegend, Cat: 104412, Clone: MEL-14), CD80 (BD Biosciences, Cat: 740130, Clone: 16-10A1), mouse I-A/I-E (MHC Class II) (Thermofisher, Cat: 64-5321-80, Clone: M5/114.15.2), mouse CD274 (PD-L1) (BioLegend, Cat: 124312, Clone: 10 F.962), Ly6C (BioLegend, Cat: 128029, Clone: HK1.4), mouse CD11c (BioLegend, Cat: 117316, Clone: N418), mouse Ly6G (Biolegend, Cat: 127623, Clone: 1A8), mouse CD103 (BioLegend, Cat: 121426, Clone: 2E7), mouse F4/80 (BioLegend, Cat: 123127, Clone: BM8), mouse IL-12/ IL-23 p40 (Thermofisher, Cat: 12-7123-41, Clone:17.8), Ep-CAM/CD326 (BioLegend, Clone: 9C4) (Cat: 324208), human IL-12 p40/p70 (BD Biosciences, Cat: 554575, Clone: C11:5), biotinylated Protein L (GenScript USA, Cat: M00097) (25), TAG72 (Novus Biologicals), Clone, Cat: NBP2-33128, muCC49), Donkey Anti-Rabbit Ig (Invitrogen, Cat: A31573), Goat Anti-Mouse Ig (BD Biosciences, Cat: 550589), and streptavidin (BD Biosciences, Cat: 349023, 1:20 dilution) were used.

Techniques: